A newly modified QuEChERS method for the analysis of organochlorine and organophosphate pesticide residues in fruits and vegetables.

Abstract

A newly modified QuEChERS method was developed, validated, and tested in-house for the multiclass, multiresidue determination of organophosphate (OPP) and organochlorine pesticide (OCP) residues in fruits and vegetables. The method incorporated a solvent extraction with acetonitrile followed by partitioning with magnesium sulphate (MgSO4) and sodium chloride (NaCl). The final step involved a dispersive solid phase extraction (d-SPE) clean-up prior to gas chromatography with electron capture detection (GC-ECD) analysis. Apples and lettuce were the selected matrices for fruits and vegetables, respectively in the method development stages. Various combinations of sorbents were tested in the clean-up step. Florisil and MgSO4 (FM) d-SPE proved to be the best combination for the clean-up step for both OCPs and OPPs in both the fruit and vegetable matrices. Recovery values fell within the acceptable range of 70 to 120% (RSD ≤20%). The new method is unique as it employs the standard addition calibration technique for the quantification of OCP and OPP residues. Limit of detection, limit of quantification, linearity, recovery analysis, and repeatability were validation parameters used to evaluate the accuracy, precision, and ruggedness of the method. The newly modified QuEChERS method provides a cheaper alternative for the analysis of pesticide residues in fruits and vegetables as samples can be analysed at a fraction of the cost of the original QuEChERS method. The efficacy of the method was tested on several fruits and vegetables from the Central Division of Trinidad and Tobago. OCP and OPP residues were found in 61% of the samples, most of which were above the maximum residue limits.