Abstract
Objective: The objective of the present work is to develop and validate a new UV derivative spectrophotometric method for simultaneous estimation of metoprolol succinate and ramipril in methanol: water (50:50v/v).
 Methods: “Zero crossing technique” was chosen for quantitative determination. The zero-crossing points (ZCP’s) were found to be 209 nm where metoprolol succinate was quantified and 211 nm where ramipril was quantified. This method was then subjected to accuracy, linearity, sensitivity and reproducibility according to ICH guidelines to ensure and confirm its validity.
 Results: The method was found to be obeying Beer’s law in the range of 10-50 µg/ml and 5-25 µg/ml for metoprolol succinate and ramipril, respectively. The % recoveries were observed between the range of 99.2-100.2 for metoprolol succinate and 99.57-99.86 for ramipril. The intra-day and inter-day results showed reproducibility.
 Conclusion: It can be concluded that the developed third-order UV derivative spectroscopic method for the simultaneous determination of metoprolol succinate and ramiprilcan be recommended for routine quantitative analysis.
Highlights
Metoprolol succinate is chemically known as 1-[4-(2-methoxyethyl) phenoxy]-3-(propan-2-ylamino) propan-2-olbutanedioate
Few chromatographic methods have been mentioned in the literature to date for the simultaneous estimation of metoprolol succinate and ramipril in their binary mixtures [6, 8, 15]
One spectrophotometric method, based on simultaneous equation has been reported for combination of metoprolol succinate, atorvastatin calcium and ramipril [16]
Summary
Metoprolol succinate is chemically known as 1-[4-(2-methoxyethyl) phenoxy]-3-(propan-2-ylamino) propan-2-olbutanedioate (fig. 1). Metoprolol succinate is chemically known as 1-[4-(2-methoxyethyl) phenoxy]-3-(propan-2-ylamino) propan-2-olbutanedioate It competes with adrenergic neurotransmitters such as catecholamines for binding at β1-adrenergic receptors in the heart that result in a decrease in heart rate, blood pressure and cardiac output [1, 2]. 3,3a,4,5,6,6a-hexahydro-2H-cyclopenta[b]pyrrole-2-carboxylic acid, acts by inhibiting angiotensin-converting enzyme (ACE), thereby lowers the production of angiotensin II and decreases the breakdown of bradykinin. The decrease in angiotensin II results in lowering total peripheral resistance, widening the blood vessels and decreases the blood pressure [3]. Commercial brands of metoprolol succinate and ramipril are available and have been prescribed to the patients who are suffering from myocardial infarction, nephropathy, angina and congestive heart failure [4]
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