Abstract
A major glycosphingolipid in rat bone marrow cells was purified, and its structure was studied. The glycolipid was found to exhibit blood group B activity by the hemagglutination inhibition test. The structure was determined to be (formula; see text) by studies of nuclear magnetic resonance, sequential hydrolysis by exoglycosidases, linkage analysis of methylated sugars by gas chromatography-mass spectrometry, and immunological tests. The blood group B active glycolipid was detected not only in the bone marrow cells but also in spleen, thymus, and rat ascites hepatoma AH 7974F cells. Besides the glycolipid, gangliotriaosylceramide, gangliotetraosylceramide, and fucogangliotetraosylceramide were commonly detected in these cells. The similarity between the glycolipid species on the cell surfaces of the immunocytes and the tumor cells is discussed with the respect to an escape mechanism of the tumor cells from the immunosurveillance system.
Highlights
From the $Department of Biochemistry, Shizuoka College of Phurmmy, Shizuoka 422 Japan andthe §Biochemical Laboratory, Cancer Institute, Hokkaido University, Schoolof Medicine, Sapporo 060 Japan
Fuca 1 bone marrow cells, we found a blood group B active glycolipid by studies of nuclear magnetic resonance, sequential hydrolysis by exoglycosidases, linkageanalysis of methylated sugars by gas chromatography-massspectrometry, and immunologicaltests
The lipid was detected in spleen, thymus, andrat ascites hepatoma AH 7974Fcells
Summary
A major glycosphingolipid in rat bone marrow cells Much attentionhas been focused on Gg4Cer since the was purified, and its structure was studied. The gly- glycolipidwas proposed to be a surface marker of natural colipid was found to exhibit blood group B activity by killer cells [4,5]. Gg&er seems to be a common glycolipid the hemagglutination inhibition test. The structure in immunocytes such as murine thymus cells [6], splenic T-. Was determined to be cells [7], macrophages [8], and rat bone marrow cells [1,3]. Gal(al-3)Gal(gl-3)GalNAc~l-4)Gal(~l-4)Glc(~l-l)ceramidIen addition to Gg,Cer, glycosylated forms of Gg4Cer such as. Fuc-Gg4Cerand GMLb seemed to be useful markers
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