Abstract
Stomach adenocarcinoma (STAD) is one of the most malignant cancers that endanger human health. There is growing evidence that competitive endogenous RNA (ceRNA) regulatory networks play an important role in various human tumors. However, the complexity and behavioral characteristics of the ceRNA network in STAD are still unclear. In this study, we constructed a ceRNA regulatory network to identify the potential prognostic biomarkers associated with STAD. The expression profile of lncRNA, miRNA, and mRNA was downloaded from The Cancer Genome Atlas (TCGA). After performing bioinformatics analysis, the CCDC144NL-AS1/hsa-miR-145-5p/SERPINE1 ceRNA network associated to STAD prognosis of STAD was obtained. The CCDC144NL-AS1/SERPINE1 axis in the ceRNA network was identified by correlation analysis and considered as a clinical prognosis model by Cox regression analysis. In addition, methylation analysis indicated that the abnormal upregulation of CCDC144NL-AS1/SERPINE1 axis might be related to the aberrant methylation of some sites, and immune infiltration analysis suggested that CCDC144NL-AS1/SERPINE1 axis probably influences the alteration of tumor immune microenvironment and the occurrence and development of STAD. In particular, the CCDC144NL-AS1/SERPINE1 axis based on the ceRNA network constructed in the present study might be an important novel factor correlating with the diagnosis and prognosis of STAD.
Highlights
Gastric carcinoma (GC), stomach adenocarcinoma (STAD), continues to be of major significant cancer worldwide
The intracellular localization of DElncRNAs was investigated using the lncALTAS database because Long non-coding RNA (lncRNA) can only function as nodes of the competitive endogenous RNA (ceRNA) network in the cytoplasm
The miRNAs that matched with DElncRNAs were selected to predict mRNAs
Summary
Gastric carcinoma (GC), stomach adenocarcinoma (STAD), continues to be of major significant cancer worldwide. STAD is diagnosed by endoscopic examination and staged by CT, endoscopic ultrasound, PET, and laparoscopy. The primary treatment for early-stage STAD is endoscopic resection. Non-early operable STAD is treated with surgery, including D2 lymphadenectomy [3]. Risk factors for gastric cancer include many nonmodifiable variables, such as age, gender, race/ethnicity, and other controllable risk factors such as Helicobacter pylori infection, smoking, high nitrate, and high nitrite diet [4, 5]. The incidence and mortality of STAD continue to increase in many countries, and it is considered to be cancer with a poor prognosis and low survival rate. It is necessary to explore useful prognostic biomarkers and/or therapeutic targets for STAD to improve our deficiencies in the diagnosis, prevention, and treatment of the disease
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