Abstract
The effects of different initial sucrose concentrations and temperatures on gellan biosynthesis by Sphingomonas paucimobilis ATCC 31461 were investigated. Lower sucrose concentrations and higher temperatures were favorable for cell growth. Higher sucrose concentrations and lower temperatures promoted gellan production but retarded cell growth. Based on these results, a two-stage culture strategy was developed to improve gellan production. During the first 24h, S. paucimobilis was cultured in a pulse fed-batch mode with an initial sucrose concentration 10g/L. Ten grams per liter of sucrose were added at 12h and 24h, and the temperature was controlled at 33°C. Batch culture was performed, and the temperature was reduced to 28°C to achieve a high gellan accumulation. The two-stage culture strategy achieved the highest gellan production (22.61g/L) at 60h that was 35.71% higher than the result of the best conventional batch operation (16.66g/L). Meanwhile, high gellan yield was related to high UDPG-pyrophosphorylase activity and glucosyltransferase activity.
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