Abstract
A coal-vitamin medium was developed to isolate actinomycetes from soil, which was superior to other currently used media. It increased the number of actinomycetes and inhibited the growth of other soil bacteria. The pretreatment of soil suspension with peptone (6%) and lauryl sulfate (0.05%) at 50°C for 10 min, also greatly increased the number of actinomycetes from soil prior to incubation with new medium.
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