Abstract
BackgroundGenomic libraries of introgression lines (ILs) consist of collections of homozygous lines with a single chromosomal introgression from a donor genotype in a common, usually elite, genetic background, representing the whole donor genome in the full collection. Currently, the only available melon IL collection was generated using Piel de sapo (var. inodorus) as the recurrent background. ILs are not available in genetic backgrounds representing other important market class cultivars, such as the cantalupensis. The recent availability of genomic tools in melon, such as SNP collections and genetic maps, facilitates the development of such mapping populations.ResultsWe have developed a new genomic library of introgression lines from the Japanese cv. Ginsen Makuwa (var. makuwa) into the French Charentais-type cv. Vedrantais (var. cantalupensis) genetic background. In order to speed up the breeding program, we applied medium-throughput SNP genotyping with Sequenom MassARRAY technology in early backcross generations and High Resolution Melting in the final steps. The phenotyping of the backcross generations and of the final set of 27 ILs (averaging 1.3 introgressions/plant and covering nearly 100 % of the donor genome), in three environments, allowed the detection of stable QTLs for flowering and fruit quality traits, including some that affect fruit size in chromosomes 6 and 11, others that change fruit shape in chromosomes 7 and 11, others that change flesh color in chromosomes 2, 8 and 9, and still others that increase sucrose content and delay climacteric behavior in chromosomes 5 and 10.ConclusionsA new melon IL collection in the Charentais genetic background has been developed. Genomic regions that consistently affect flowering and fruit quality traits have been identified, which demonstrates the suitability of this collection for dissecting complex traits in melon. Additionally, pre-breeding lines with new, commercially interesting phenotypes have been observed, including delayed climacteric ripening associated to higher sucrose levels, which is of great interest for Charentais cultivar breeding.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0842-0) contains supplementary material, which is available to authorized users.
Highlights
Genomic libraries of introgression lines (ILs) consist of collections of homozygous lines with a single chromosomal introgression from a donor genotype in a common, usually elite, genetic background, representing the whole donor genome in the full collection
We report the development of a new IL population derived from the cross between the French cultivar Vedrantais (VED), a Charentais type, as recurrent parent and the Japanese Ginsen makuwa (MAK) cultivar
Fifteen BC1 plants derived from the cross VED x MAK were backcrossed to the recurrent parent, producing fifteen BC2 families, each one with twenty eight plants
Summary
Genomic libraries of introgression lines (ILs) consist of collections of homozygous lines with a single chromosomal introgression from a donor genotype in a common, usually elite, genetic background, representing the whole donor genome in the full collection. Repeats) collections (available at the [4]), which have facilitated the construction of consensus saturated maps [5, 6] Some of these collections have been implemented in high-throughput genotyping platforms and have been used for genetic diversity and association studies [7, 8]. Some of these markers have been used to anchor genetic and physical maps [6] on the melon genome sequence [9,10,11]. These tools have allowed the genetic dissection of both simple and complex traits [18,19,20,21,22,23,24,25,26]
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