Abstract
Coriandrum sativum an important spice exhibits lower seed germination. That may be due to phenolic compounds that induce dormancy in seeds. This study aimed to develop a comprehensive seed enhancement technique to overcome dormancy and improve germination and seedling growth. Seeds were subjected to GC-MS analysis to identify bioactive components. Seeds were leached for 6 to 30 h (Leaching). Following leaching, hydropriming for 3 to 18 h to standardize the priming duration. At the standardized priming duration, seeds were primed with hydrogen peroxide, nitric oxide, salicylic acid and 2,4-D (leaching + priming). Additionally, leached seeds were coated with polymer and plant growth-promoting microorganisms such as Azospirillum, Trichoderma viride and Bacillus subtilis (leaching + coating). Finally, the best-performing treatments from these experiments were compared with comprehensive technique. The results revealed that coumarin was the primary inhibitor. Leaching (24 h) significantly improved germination by 25.8 % over control. The duration of priming was standardized to 6 h after leaching. Leaching (24 h) + priming with 25 mM H2O2 (6 h) significantly increases germination percentage by 30.7 % over control. Leaching (24 h) + coating with polymer and Azospirillum at 100 g/kg of seeds significantly increased germination by 32.8 % over control. Assessment of the comprehensive techniques revealed that seed leaching (24 h) + priming with 25 mM H2O2 (6 h) + coating with polymer and Azospirillum at 100 g/kg significantly increased the germination percentage by 39 % over control. Results show that a comprehensive technique breaks dormancy and improves germination and seedling growth.
Published Version
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