Abstract
In the present work, monolayer engineering was used to fabricate biocatalyticnanostructured thin films based on the enzyme penicillin G acylase. Thebiocatalytic films with enhanced characteristics were produced by the depositionof alternate-layer assemblies with a predetermined structure using a combinationof Langmuir–Blodgett and adsorption techniques. The value of enzyme activityand the level of protein detachment were measured in dependence on the variationof film composition and on the sequence of layer alternation. As a result, highlyactive and stable structures were found, which could be promising candidates forpractical applications. The method of modification of the deposition method toprovide continuous film formation on large-area supports is discussed.
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