Abstract
The cell microenvironment plays a pivotal role in mediating cell adhesion, survival, and proliferation in physiological and pathological states. The relevance of extracellular matrix (ECM) proteins in cell fate control is an important issue to take into consideration for both tissue engineering and cell biology studies. The glycosylation of ECM proteins remains, however, largely unexplored. In order to investigate the physio-pathological effects of differential ECM glycosylation, the design of affordable chemoselective methods for ECM components glycosylation is desirable. We will describe a new chemoselective glycosylation approach exploitable in aqueous media and on non-protected substrates, allowing rapid access to glyco-functionalized biomaterials.
Highlights
Tissue engineering relies on the possibility to engineer cell microenvironments by means of bioactive materials providing biochemical and physical stimuli to guide cell behavior and to regenerate damaged tissue [1,2,3]
Collagen 2D films neoglycosylated with glucose are able to drive F11 neuroblastoma cell line from proliferation to differentiation into functionally active neurons [7]
Maltose and glucose were conjugates to N-methoxyamino-collagen films and the final neoglycosylated materials were characterized in terms of chemical and morphological features
Summary
Tissue engineering relies on the possibility to engineer cell microenvironments by means of bioactive materials providing biochemical and physical stimuli to guide cell behavior and to regenerate damaged tissue [1,2,3]. Bioactive materials can be designed and formulated by the incorporation of specific biological signals, using both peptides or glycans able to control cell adhesion or differentiation. Glycans represent an interesting class of biomolecules due to their ubiquity in tissues, and more importantly, due to their functional and structural roles in cell development. Glycosylated collagen 2D films, obtained via reductive amination of a glycan with the collagen lysins, showed that glycosylation of the biomaterials influences the cell fate [6]. Collagen 2D films neoglycosylated with glucose are able to drive F11 neuroblastoma cell line from proliferation to differentiation into functionally active neurons [7]. Reductive amination reaction for neoglycosylation of collagen. Data are mean ± SD and compared by Student’s t-test (* p < 0.05)
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