Abstract
Seventeen strains of flexibacteria associated with fish disease were evaluated with the API 20E system of biochemical tests. The results differentiate between isolates from warmwater fish, which utilize arabinose, melibiose, and sucrose; and isolates from cold-water fish, which are negative on these tests. Cold-water fish isolates may be further divided into subgroups on the basis of gelatin hydrolysis. The API system appears to provide the first biochemical differentiation of this group of poorly defined organisms.Key words: API 20E, flexibacteria, differentiate
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