A new antileishmanial compound, phaseolinone

Abstract

Inclusion of phaseolinone, a newly described mycotoxin, at 20 μg per ml in a solid culture medium (blood agar overlay) and at 50 μg per ml in a liquid culture (medium 199) inhibited the growth of L . donovani promastigotes. About 90% of the motile promastigotes lost motility after exposure to 50 μg per ml of phaseolinone for 6–7 h and here 3-day-old culture was more sensitive than 7-day-old culture. In an in vitro assay, DNA dependent RNA polymerase activity of 3-day-old promastigotes was considerably inhibited in the presence of this toxin. Therefore, this key enzyme was suggested to be one of the sites of action of phaseolinone.