A neutral polysaccharide from the abalone pleopod, Haliotis discus hannai Ino

Abstract

For the first time, a water-soluble neutral polysaccharide, APP, was obtained from abalone pleopod (Haliotis Discus Hannai Ino) by proteolytic method with papain followed by precipitation with ethanol and purification with gel chromatography. The results of HPLC with TSK-gel column and gel filtration chromatography with Sepharose CL-6B indicated APP was uniform in polarity and its molecular weight (MW) was about 6 kDa. According to chemical methods, IR spectroscopy, and NMR spectroscopy, APP consists of 1-, 1,4-, 1,6-, 1,4,6-linked glucose and 1-, 1,3-, 1,6-, 1,4,6-linked galactose.