Abstract
Abstract Wide compatibility and erect panicles are important traits for heterosis and ideal plant type breeding in rice and accordingly are targets for rice yield improvement. In this study, a multiplex PCR system based on functional markers was developed to simultaneously identify genotypes of the wide compatibility allele S5-n and the erect panicle allele dep1. Forty-nine rice varieties in the Huang-Huai-Hai region of China were identified using this system. Thirty-three varieties contained the erect panicle allele dep1 and two varieties contained the wide compatibility allele S5-n. The frequency of dep1 was obviously higher than that of S5-n. The multiplex PCR method was also tested using an F2 segregating population and was found to be simple, efficient, and reliable. It will be useful in marker-assisted selection based on the wide compatibility allele S5-n and erect panicle allele dep1 to breed super rice varieties using indica and japonica heterosis.
Highlights
Rice is one of the most important food crops worldwide
According to Chen et al (2001), rice yield improvement in the future will depend on super high-yield rice breeding that combines ideal plant type and heterosis
The results indicated that the system could be applied to the rapid detection of S5-n and dep1 in rice germplasm resources and to molecular marker-assisted selection in indica-japonica hybrid breeding
Summary
Rice is one of the most important food crops worldwide. The continuous improvement of rice yield is an important way to ensure food security (Liang et al 2014). According to Chen et al (2001), rice yield improvement in the future will depend on super high-yield rice breeding that combines ideal plant type and heterosis. Two subspecies of Asian cultivated rice (Oryza sativa L.), show strong F1 heterosis, and have the potential to produce super hybrid rice plants (Konishi et al 2006, Qian et al 2016). Overcoming the problem of hybrid sterility between indica and japonica to improve yield is an important topic in rice breeding research
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