Abstract
Confocal microscopy is a well-established standard for biological fluorescence imaging, capable of providing high contrast and axial sectioning through rejection of out of focus light. However, typical confocal techniques can suffer from slow imaging speeds, excessive bleaching, or small fields of view which make it challenging to image large areas at high resolution efficiently. Here we report on a custom-built line scanning confocal microscope with two opposing views that is capable of quickly imaging samples up to several millimeters in length at high resolution.
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