Abstract
A monoclonal antibody, referred to as 38.13, was obtained by fusing murine myeloma cells with Lewis rat splenocytes sensitized with Daudi cells (human Burkitt lymphoma). 38.13 antibody was demonstrated to be a rat IgM. By complement dependant microcytotoxicity and indirect immunofluorescence assays, 38.13 antibody was shown to react specifically with cells derived from Burkitt tumors, including both Epstein-Barr virus genome carrying and Epstein-Barr virus negative Burkitt lymphoma. By contrast Epstein-Barr virus containing lymphoblastoid cell lines, derived from normal B lymphocytes were not recognized by 38.13 antibody. Malignantfresh cells from patients affected with various lymphoproliferative disorders were negative, except 4/8 cases of abdominal Burkitt-like lymphomas. Normal lymphocytes from peripheral blood, spleen, lymph node, tonsil and bone-marrow and mitogen (PHA, PWM and Con A) activated blasts were also negative. Thus 38.13 antibody apparently recognized a Burkitt associated antigen, which is clearly not related to Epstein-Barr virus.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
