Abstract
A mini-trough for the study of membrane insertion ability of proteins was designed and constructed. This trough was made up of teflon, small and handy, having 1.5 ~ 2.0 ml subphase volumes and 6 cm 2 surface areas for the monolayer spreading. With the unique construction, the vortex flow of fluids driven by the magnetic stir bar in the trough is converted into the laminar flow, which has no influence on the monolayer stability and surface pressure measurement. So the fluctuations of monolayers spread at the surface of subphase are small and the protein samples injected into the subphase could rapidly mix with the bulk solution, so as to make the response time is much rapid. Using this mini-trough, membrane insertion ability of melittin and apocytochrome c was studied.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
