Abstract
An IBM-PC based software system to quantify images of biological sytems is presented. To illustrate the potential of the imaging system described, UV light-induced chlorophyll fluorescence of Chlorella vulgaris cells was monitored using a video camera interfaced to a microscope. The photoinhibitor, DCMU (30 μM) inhibited the UV-induced fluorescence decay in heterotrophically grown cells but not in autotrophically grown cells. Algal cells were also incubated in various concentrations of ascorbic acid (500, 250, 100, 50, 10 and 0 mM) prior to UV light exposure. In the presence of 500, 250 and 100 mM ascorbic acid, the decay of chlorophyll fluorescence was significantly delayed in both heterotrophically and autotrophically grown cells. Heterotrophically grown cells were more sensitive to ascorbic acid than autotrophically grown cells since 10 nM ascorbic acid delayed fluorescence decay in heterotrophic cultures.
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