Abstract
Abstract Biotinylated single base extension (SBE) products that can fluoresce through the successful incorporation of fluorophore-labeled dideoxynucleoside triphosphate were immobilized on streptavidin-coated magnetic bead surfaces via the biotin–streptavidin interaction. The resultant beads were washed and packed into a capillary, followed by fluorescence imaging for detection. Using the above strategy, our bead-based SBE assay could clearly identify the allelic compositions at the targeted site on a tested DNA sequence.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Paper version not known
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
