Abstract
AbstractThere is great interest in the isolation and concentration of ω‐3‐polyunsaturated fatty acids because of their role in coronary heart diseases [1–3]. For that reason suitable methods for determination of such fatty acids are necessary.The analytical determination of ω‐3‐docosahexaenoic and ω‐3‐eicosapentaenoic acid in complex mixtures of unsaturated fatty acids like marine lipids by packed column gaschromatography [4] will not be succesfull, if you want to avoid preseparations [5, 6]. Good results can be achieved by means of capillary gaschromatography [7, 8] and high performance liquid chromatography [9]. In the cases of enrichment it is sufficient to know only the total content of ω‐3‐fatty acids.By means of reductive ozonolytical cleavage used for exploring fatty acids structures [10, 11] every fatty acid family gives characteristic cleavage products at their alkyl ends: ω‐3 = propanal; ω‐6 = hexanal; ω‐9 = nonanal [12, 13]. Taking that fact in consideration we developed a simple method for the quantitative determination of total ω‐3‐fatty acid content in lipids.
Published Version
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