Abstract
We present a method for screening lipid-soluble arsenic compounds (arsenolipids) in fish oils by reversed-phase HPLC-ICPMS using a gradient elution with ethanol and acetate buffer at pH 6. Two different approaches were tested to reduce changes in arsenic response due to the carbon effect: addition of a supplementary methanol solution directly to the spray chamber or addition of methanol post-column through a T-piece. The latter method proved to be the best option for maintaining constant response for several arsenolipids covering a wide range of polarities. With the optimized method it is possible to perform a screening of at least three groups of arsenolipids with different polarities in 90 min with detection limits ranging from 5 to 11 μg As L−1, depending on the analyzed compound. The method was applied to the screening of arsenolipids in fractions obtained from cod liver oil and capelin oil, which include arsenic-containing fatty acids, arsenic-containing hydrocarbons and another group of lower polarity and unknown character.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
