Abstract

A method for collecting microbe-free Amyloodinium ocellatum from infected red drum ( Sciaenops ocellatus) is described. Surface contamination of trophonts removed from fish by osmotic shock was reduced by washing the preparation with antibiotic containing saltwater and Percoll® density gradient purification. The resulting tomont preparation was free of external contamination. Sterile, infective dinospores excysted from the tomonts after 72 h incubation at 26°C in antibiotic containing 30 ppt saltwater.

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