Improved cell culture propagation of Amyloodinium ocellatum

Abstract

DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsSpecials DAO 24:173-178 (1996) - doi:10.3354/dao024173 Improved cell culture propagation of Amyloodinium ocellatum Oestmann DJ, Lewis DH A system for cultivating Amyloodinium ocellatum was developed using aggregates of red drum Sciaenops ocellatus dorsal fin cells in suspension. Tomonts harvested from infected fish using density gradient centrifugation in Percoll were placed in saltwater media (30 ppt) containing anti-biotics (penicillin 100 IU ml-1, streptomycin 100 mg ml-1). Microbe-free infective dinospores emerging from the tomonts were used to infect red drum cell aggregates. Dinospores attached and transformed into trophonts on the cell aggregates, developing into normal size (80 um) trophonts in saltwater media (10 ppt). Trophont infected cell aggregates were placed in increased saltwater concentration (30 ppt) at 48 h incubation to induce the release of trophonts and their encystment as tomonts. Tomonts were separated from the cellular debris by saltwater media washing and Percoll density gradient centrifugation. Tomonts were then incubated to produce a second generation of dinospores. The parasite was maintained in vitro for 10 complete life cycles with no observed decreased viability using this system. Amyloodinium . Protozoa . Propagation . Cell culture Full text in pdf format PreviousNextExport citation RSS - Facebook - Tweet - linkedIn Cited by Published in DAO Vol. 24, No. 3. Publication date: March 07, 1996 Print ISSN:0177-5103; Online ISSN:1616-1580 Copyright © 1996 Inter-Research.