A Method for Determining Ascaris Viability Based on Early-to-Late Stage In-Vitro Ova Development

Abstract

This study suggests a new method for determining the viability of Ascaris spp. ova, based on in-vitro early-to-late stage development of ova. This method includes stages prior to larval development, providing an estimation of potential viability. After application of biosolids onto soil and exposure to 7°C, 22°C, or 37°C for 45 days, ova were microscopically distinguished as viable or non-viable according to progression through development categories. Results were compared to viability estimates from current methods that distinguish viable ova as motile larva. Results suggest conventional techniques underestimate viability, whereas the new method provides a more conservative approach.