Abstract

Cl − HCO 3 − exchange transport in the glial plasma membrane is remarkably similar to anion exchange transport in erythrocytes. When LRM55 cells were pretreated with the extracellular labeling reagent [ 125I]iodosulfanilic acid, radioactivity appeared primarily in two bands on denaturing polyacrylamide gels at M r 70,000 and 30,000. Antibody to the human erythrocyte anion exchanger (Band 3) cross-reacted on immunoblots with an LRM55 band at M r 68,000. The LRM55 Cl − HCO 3 − exchanger may be a component of M r 68,000 to 70,000.

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