Abstract
Casticin, chrysoplenol-D, p-hydroxy benzoic acid, and p-methoxy benzoic acid are representative flavonoids and benzoic acid derivatives in Vitex trifolia, a well-known traditional Chinese, as well as ayurvedic medicine, with a wide range of biological activities. In the present paper, the aforementioned four markers were isolated and simultaneously quantified by TLC densitometric method. In the present study, a novel stability-indicating high-performance thin-layer chromatography (HPTLC) method for quantitative determination of the aforementioned four markers has been developed and validated on silica using solvent dichloromethane–methanol–formic acid (9.6:0.4:0.06 v/v/v) in the absorbance mode at 254 nm. The method showed a good linear relationship (r2 = 0.999) in the concentration range 100–2400 ng per band. It was found to be linear, accurate, precise, specific, robust, and stability-indicating and can be applied for quality control and standardization of other multi-component formulations based on Vitex trifolia.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
