A Lower Proportion of Regulatory B Cells in Patients with Henoch-Schoenlein Purpura Nephritis.

Xintong Hu,Zhihui Qu,Jiandong Tai,Yanfang Jiang,Man Li,Li Zhang,Songchen Zhao,Xiguang Sun,Menno C Van Zelm

doi:10.1371/journal.pone.0152368

Xintong Hu, Zhihui Qu + Show 7 more

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https://doi.org/10.1371/journal.pone.0152368

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Journal: PloS one	Publication Date: Mar 31, 2016
Citations: 15	License type: CC BY 4.0

Affiliation: First Hospital of Jilin University, Jilin University

Abstract

BackgroundHenoch—Schoenlein purpura is the one of most common types of systemic vasculitis that involves impaired renal function and Henoch-Schoenlein purpura nephritis (HSPN). The diagnosis of this condition is largely based on immunohistologic detection of immunoglobulin A1-containing immune complex in the glomerular deposits of mesangium. Despite clinical advances, the etiopathogenesis of HSPN is still largely unknown.MethodsIn this study, we enrolled 25 newly diagnosed HSPN patients and 14 healthy controls. Then, fractions of B cell subtypes were determined in venous blood using flow cytometry. The serum interleukin (IL)-10 concentration was determined by enzyme-linked immunosorbent assay.ResultsCompared to those in healthy controls, the numbers of CD38+CD19+, CD86+CD19+, CD38+CD86+CD19+, and CD95+CD19+ B cells per microliter of blood were significantly higher in HSPN patients. In contrast, the numbers of CD5+CD19+, IL-10+CD19+, CD5+CD1d+CD19+, and IL-10+CD5+CD1d+CD19+ B cells per microliter of blood and the serum IL-10 concentration were significantly lower in HSPN patients. Following treatment, the numbers of CD38+CD19+ and CD86+CD19+ B cells per microliter of blood were significantly reduced in HSPN patients. However, the numbers of CD5+CD1d+CD19+, CD5+CD1d+IL-10+CD19+, and IL-10+CD19+ B cells per microliter of blood and the serum IL-10 concentration were significantly increased in HSPN patients following treatment. The estimated glomerular filtration rate (eGFR) was negatively correlated with the number of CD38+CD19+ B cells but positively correlated with the numbers of IL-10+CD19+, CD1d+CD5+CD19+, and IL-10+CD1d+CD5+CD19+B cells per microliter of blood and the serum IL-10 concentration. The 24-h urinary protein concentration was positively correlated with the number of CD38+CD19+B cells but negatively correlated with the numbers of IL-10+CD19+, CD1d+CD5+CD19+, and IL-10+CD1d+CD5+CD19+B cells per microliter of blood and the serum IL-10 concentration.ConclusionOur results suggest that CD38+CD19+ and CD1d+CD5+CD19+ B cells (Bregs) contribute to the pathogenesis of HSPN.

Highlights

Henoch—Schoenlein purpura (HSP) is a systemic vasculitis that affects small vessels
Compared to those in healthy controls, the numbers of CD38+CD19+, CD86+CD19+, CD38+CD86+CD19+, and CD95+CD19+ B cells per microliter of blood were significantly higher in Henoch-Schoenlein purpura nephritis (HSPN) patients
Our results suggest that CD38+CD19+ and CD1d+CD5+CD19+ B cells (Bregs) contribute to the pathogenesis of HSPN

Summary

Introduction

Henoch—Schoenlein purpura (HSP) is a systemic vasculitis that affects small vessels In this condition, patients develop perivascular inflammatory cell infiltrates. The CD95 receptor is considered to be a key regulator in the activation of germ cell apoptosis [13] These different subtypes of B cells are known to collaborate and control the responses of the human immune system; very little information is available regarding the mechanisms governing the onset of HSPN in patients. Henoch—Schoenlein purpura is the one of most common types of systemic vasculitis that involves impaired renal function and Henoch-Schoenlein purpura nephritis (HSPN) The diagnosis of this condition is largely based on immunohistologic detection of immunoglobulin A1-containing immune complex in the glomerular deposits of mesangium. The etiopathogenesis of HSPN is still largely unknown

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Conclusion