Abstract
A lateral flow immunochromatographic strip test (LFIST) based on a competitive format was developed for rapid and sensitive on-site detection of oseltamivir phosphate (OP) residues in poultry product. The sensitivity (half inhibitory concentration, IC50) of the LFIST in the detection of egg and chicken meat samples was confirmed to be 2.56 and 2.63 µg/kg, and the limit detection (LOD) value were 0.43 and 0.42 µg/kg, respectively. For intra-assay and inter-assay reproducibility, recoveries of OP spiked samples ranged between 82.8% and 91.2% with coefficients of variations (CV) less than 5.67% (intra-assay) and 6.52% (inter-assay). The performance of LFIST was comparable to high-performance liquid chromatography (HPLC) in a parallel testing of egg samples and chicken samples. LFIST takes less than 5 minutes, eliminates the dependency on professional personnel, and thus can be used as a surveillance tool for on-site detection of OP residues.
Highlights
Oseltamivir phosphate (OP) is an ethyl ester prodrug widely used to treat and prevent infections from influenza A and B virus[1,2]
In ELISAs were synthesized by coupling free amino groups of the carrier proteins and carboxyl acid group of the oseltamivir carboxylic acid (OCA) using the active ester method (Fig. 2)
The use of oseltamivir phosphate (OP) as a veterinary drug has been banned, illegal and even excessive use occurs in cases, which poses a threat to public health in developing countries
Summary
Oseltamivir phosphate (OP) is an ethyl ester prodrug widely used to treat and prevent infections from influenza A and B virus[1,2]. Several analytical methods for the detection of OP have been reported, including HPLC16,17, ultra-high- performance liquid chromatography-tandem mass spectrometry (UPLC–MS/MS)[18,19], and liquid chromatography-mass spectrometry(LC-MS)[20]. These methods are highly accurate and sensitive, they are dependent on expensive analytical instruments, complex pre-treatment steps, and skilled professionals. They are not suitable for high-throughput assay and real time on-site detection. It showed that the LFIST was OP-specific and could provide qualitative results with naked eyes within 5 min and quantitative results with the aid of a strip reader
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