Abstract
A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg−1, and the limit of detection value was 0.62 µg kg−1. For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91–4.64% and 4.24–5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.
Highlights
Hexoestrol (HES, 4,4’-(1,2-diethyl-1,2-ethanediyl) bisphenol), known as hexoestrolum, is a kind of synthetic non-steroidal oestrogen, which can maintain the female character of the organism, promote the growth and development of reproductive organs, and adjust a positive and negative feedback on the hypothalamus and pituitary gland [1,2]
We describe the development of an lateral flow immunochromatographic strip test (LFIST) for the rapid detection of HES residues in fish using an anti-HES monoclonal antibody
enzyme-linked immunosorbent assay (ELISA) plates and culture plates were bought from Nunc (Roskilde, Denmark); nitrocellulose (NC) membrane, glass fibre and absorbent pad were purchased from Millipore (Bedford, MA, USA); 4-bromobutyric acid ethyl ester, Goat anti-mouse IgG antibody (GaMIgG-HRP), N, N-dimethylformamide (DMF) (Shanghai, China); seven-week-old female BALB/c mice were acquired from the SPF standard Laboratory Animal Center (Zhengzhou University, China); GS-NS0 myeloma cells were donated by the institute for Animal Health of Great Britain; all other reagents and solvents were of analytical grade or higher in this study
Summary
Hexoestrol (HES, 4,4’-(1,2-diethyl-1,2-ethanediyl) bisphenol), known as hexoestrolum, is a kind of synthetic non-steroidal oestrogen, which can maintain the female character of the organism, promote the growth and development of reproductive organs, and adjust a positive and negative feedback on the hypothalamus and pituitary gland [1,2]. Developing new methods for monitoring HES is necessary and important
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