Abstract
Electron transfer from P700 in photosystem I (PSI) particles from spinach to Anabaena sp. PCC 7119 flavodoxin has been studied using laser flash absorption spectroscopy. A non-linear protein concentration dependence of the rate constants was obtained, suggesting a two-step mechanism involving complex formation ( k=3.6 × 10 7 M −1· s −1) followed by intracomplex electron transfer ( k=270 s −1). The observed rate constants had a biphasic dependence on the concentrations of NaCl or MgCl 2 with maximum values in the 40–80 mM range for NaCl and 4–12 mM for MgCl 2. To our knowledge, this is the first time that the kinetics of PSI-dependent flavodoxin photoreduction have been determined.
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