A Large-Scale and Serverless Computational Approach for Improving Quality of NGS Data Supporting Big Multi-Omics Data Analyses.

Abstract

Various types of analyses performed over multi-omics data are driven today by next-generation sequencing (NGS) techniques that produce large volumes of DNA/RNA sequences. Although many tools allow for parallel processing of NGS data in a Big Data distributed environment, they do not facilitate the improvement of the quality of NGS data for a large scale in a simple declarative manner. Meanwhile, large sequencing projects and routine DNA/RNA sequencing associated with molecular profiling of diseases for personalized treatment require both good quality data and appropriate infrastructure for efficient storing and processing of the data. To solve the problems, we adapt the concept of Data Lake for storing and processing big NGS data. We also propose a dedicated library that allows cleaning the DNA/RNA sequences obtained with single-read and paired-end sequencing techniques. To accommodate the growth of NGS data, our solution is largely scalable on the Cloud and may rapidly and flexibly adjust to the amount of data that should be processed. Moreover, to simplify the utilization of the data cleaning methods and implementation of other phases of data analysis workflows, our library extends the declarative U-SQL query language providing a set of capabilities for data extraction, processing, and storing. The results of our experiments prove that the whole solution supports requirements for ample storage and highly parallel, scalable processing that accompanies NGS-based multi-omics data analyses.