Abstract
SummaryA large plaque variant of Sindbis virus was obtained by repeatedly cloning after serial passage 50 times in chorioallantoic membrane in tissue culture. This virus has a high infectious titer in CEF cells as well as suckling mice. On the other hand, a small plaque of Sindbis virus obtained by the same method above using chick embryo brains instead of chorioallantoic membrane, grows to somewhat lower titer than that of the large plaque variant, and is not sensitive to agar inhibitors.
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