Abstract
Research on the mechanism of action of cellulases has been hampered by the lack of a rapid, continuous, or kinetic assay. A linked assay system that uses glucose oxidase and horseradish peroxidase has been coupled with β-glucosidase to yield an assay system that can be used for kinetic assays for cellobiase-producing enzymes as well as a measure of cellobiose degradation by β-glucosidases. This assay shows a 20-fold increase in sensitivity over the traditional reducing sugar assay.
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