Abstract
Interaction of rabbit muscle pyruvate kinase (ATP:pyruvate 2- O-phosphotransferase, EC 2.7.1.40) with inhibitory and activatory effectors was analyzed in terms of the Yagi-Ozawa equation. Straight-line plots obtained for l-phenylalanine+ l-alanine and l-phenylalanine+ l-phenylalanyl methyl ester (PheAlaOMe) indicated competition for common sites. Parabolic plots for 2-phosphoglycerate and l-phenylalanine or PheAlaOMe suggested lack of competition between these compounds. Biphasic concave plots were obtained for 2-phosphoglycerate and l-1-amino-2-phenylethyl phosphonic acid (PnPhe), and mixtures of inhibitory ( l-phenylalanine, PheAlaOMe) and activatory (PnPhe, l-alanine and l-1-aminoethyl phosphonic acid (PnAla)) amino acid derivatives. The latter non-classical Yagi-Ozawa plots can be interpreted in terms of the existence of distinct regulatory sites for inhibitory and activatory ligands.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular Enzymology
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