Abstract
AbstractA pseudo‐first order kinetic analysis has described the reactions of purine ribonucleotide synthesis and interconversion in Ehrlich ascites tumor cells in vitro. Rate constants were determined for 13 reactions at non‐saturating precursor concentration (50 μM); nucleotide formation from adenine‐14C and hypoxanthine‐14C was rate limiting for ATP synthesis and nucleotide formation from guanine‐14C and hypoxanthine‐11C was rate limiting for GTP synthesis. The initial rites of nucleotide synthesis from 1.0 mM adenine‐14C and hypoxanthine‐14C were equivalent to the rates of nucleotide synthesis at non‐saturating base concentration, but the rate of nucleotide synthesis at saturating base concentration after 30 minutes incubation was 20‐25% that of non‐saturating precursor concentration.
Published Version
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