A human myeloma protein with specificity against dinitrophenyl and nucleic acid derivatives

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Immunochemical studies on a human IgG myeloma protein which precipitates with DNA-protein-conjugates are reported. This monoclonal immunoglobulin showed crossreactions with protein conjugates of a number of other compounds such as pyrimidines, purines, nucleotides and nucleosites. In addition it reacted with single stranded DNA and, to a much lesser extent, with native DNA. No reaction, however, was detectable with the free haptens DNA-lysine and 5-acetyluracil-1-caproic acid and with nucleotides and nucleosides. The binding activity could be localized in the Fab fragment of the immunoglobulin molecule.