Abstract
Aims: the present study mainly aimed to assess the qualitative and quantitative assessment of gallbladder mucins in chronic calculous cholecystitis, correlating the mucin histochemistry and morphology of the gallbladder in chronic calculus cholecystitis with each other and with chemical composition of gallstones. Methods: Hematoxylin and Eosin stained sections of the selected specimens were screened and one to two sections with adequate amount of well-preserved mucosa with lining epithelium were chosen. the corresponding paraffin blocks were selected and isolated. Four sections were cut on each of the selected blocks for special stains. results: comparison of inflammation and mucin histochemistry, inflammation and metaplasia, composition of calculi and grade of inflammation, composition of stones and mucin
Highlights
The three specimens of gallbladder used as a control in the present study showed predominantly Alcian blue (AB) positive mucins in the surface and deep mucosal epithelium
High iron diamine and alcian blue (HID-AB) stain was done in the three controls and it showed strong High Iron Diamine – Alcian Blue Stain (HID-AB) positivity indicating the predominance of sulfomucin in superficial and deep mucosa and evaluated by mean score which is normally observed in the normal gallbladder mucosa (Table 1)
The mean scores for AB positive in the superficial and deep mucosa, in the three grades of inflammation showed a progressive decrease for Alcian Blue positive mucins in the superficial and deep mucosal epithelium, with increasing grades of inflammation (Table 2)
Summary
A total number of 40 specimens were selected from gallbladders with clinical and histopathological diagnosis of chronic calculous cholecystitis received in the department of pathology PSG Institute of Medical Sciences and Research during the period of 2005–2007. (1) Histopathological confirmation of chronic calculous cholecystitis. Hematoxylin and Eosin (H&E) stained sections of the selected specimens were screened and one to two sections with adequate amount of well-preserved mucosa with lining epithelium were chosen. The corresponding paraffin blocks were selected and isolated. Four sections were cut on each of the selected blocks for special stains. A pro forma was prepared for assessment. The slides were assessed according to the pro forma
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