Abstract
The present study deals with the histochemical demonstration of 17β-estradiol dehydrogenase in human term placenta using the polyvinyl alcohol method to reduce diffusion artefacts. Incubations took place with both NAD+ and NADP+ as coenzymes and at different pH values of the incubation medium. The NAD+ linked enzyme reaction showed a greater activity than the NADP+ linked, both in the trophoblast as well as in connective tissue. There were differences in staining intensity at the different pH values, and strongest reaction was observed using glycine-NaOH buffer pH 10 in the incubation medium. Owing to a non-enzymatically reduction of nitro blue tetrazolium by reduced NAD+, the demonstration of 17β-estradiol dehydrogenase is independent of diaphorase at this high pH. The findings are discussed in relation to data about “nothing dehydrogenase” and biochemically determined pH optima for the enzymatic reactions dealt with in this work.
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