Abstract
A quantitative lateral-flow immunoassay using gold nanoparticles (AuNPs) conjugated with a monoclonal antibody (MAb) against saikosaponin d (SSd) was developed for the analysis of SSd. The AuNPs were prepared in our laboratory. The AuNPs were polyhedral, with an average diameter of approximately 18 nm. We used the conjugation between AuNPs and MAbs against SSd to prepare immunochromatographic strips (ICSs). For the quantitative experiment, the strips with the test results were scanned using a membrane strip reader, and a detection curve (regression equation, y = −0.113ln(x) + 1.5451, R2 = 0.983), representing the averages of the scanned data, was obtained. This curve was linear from 96 ng/mL to 150 μg/mL, and the IC50 value was 10.39 μg/mL. In this study, we bring the concept of POCT (point-of-care testing) to the measurement of TCM compounds, and this is the first report of quantitative detection of SSd by an ICS.
Highlights
Colloidal gold nanoparticles (AuNPs), defined as gold particles with diameters ranging from 5 to 250 nm, have found widespread use in biological electron microscopy [1]
The high-resolution TEM (HRTEM) image taken from an individual A quantitative lateral-flow immunoassay using gold nanoparticles (AuNPs) shows a continuous fringe pattern with a spacing of 0.117 nm
A proposed model of the AuNPs with multi-aspect optimization is depicted in Figure 1D (4)
Summary
Colloidal gold nanoparticles (AuNPs), defined as gold particles with diameters ranging from 5 to 250 nm, have found widespread use in biological electron microscopy [1]. The colloidal gold immunochromatographic assay (GICA) was developed in the 1980s to detect specific compounds in various types of samples in one step without other treatments using a cellulose membrane as the carrier [2,3,4]. Membrane-based lateral-flow immunochromatographic strips (ICSs) are a useful tool for low-cost and rapid detection based on the GICA [7,8,9]. The popularity of ICSs, especially for common tests such as for pregnancy, drugs abused, and food safety, is driven by the ease of use, speed, and relative accuracy of these simple and inexpensive disposable devices [10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
