Abstract
Abstract A 3-hydroxyflavone-based colorimetric and fluorescence probe for hydrogen sulfide (H 2 S) has been developed with high sensitivity and excellent selectivity. This probe was designed based on the mechanism that H 2 S selectively cleaved the NBD (7-nitro-2,1,3-benzoxadiazole) ether moiety in this probe and therefore release the fluorophore, 3-hydroxyflavone. The addition of H 2 S to the solution of probe 1 resulted in a green fluorescence and an obvious color change from colorless to pink, indicating that this probe can serve as a colorimetric and fluorescent dual probe for H 2 S. Furthermore, this probe displays a rapid response and reaches to a plateau within 5 min. It also exhibits a 146 nm Stokes shift and a low detection limit (20 nM, based on S/N=3) in detecting H 2 S. Importantly, practical utility of this probe for the selective detection of H 2 S in living cells has been successfully demonstrated.
Published Version
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