Abstract
A sensitive HPLC method for direct determination of allantoin in ruminal digesta, urine and blood serum of sheep is described. Blood serum and urine samples were diluted 1:3 with deionized water. Separation and quantification of allantoin was achieved using three long Nova Pac C 18 -co- lumns (Waters) and UV detection at 205 and 215 nm. The low coefficient of variation and satisfac- tory recovery of allantoin added to assayed samples (~100%) provide a suitable tool for routine quantification of allantoin in a large number of urine, ruminal digesta and blood serum samples.
Full Text 
Sign-in/Register to access full text options
Sign-in/Register to access full text options 
Paper version not known
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
