A high-density consensus linkage map of white lupin highlights synteny with narrow-leafed lupin and provides markers tagging key agronomic traits

Michał Książkiewicz,Barbara Naganowska,Sandra Rychel,Paolo Annicchiarico,Maria Carelli,Magdalena Tomaszewska,Hua’An Yang,Stanisław Stawiński,Bogdan Wolko,Matthew N Nelson,Nelson Nazzicari,Daniel Renshaw,Barbara Ferrari

doi:10.1038/s41598-017-15625-w

Abstract

White lupin (Lupinus albus L.) is a valuable source of seed protein, carbohydrates and oil, but requires genetic improvement to attain its agronomic potential. This study aimed to (i) develop a new high-density consensus linkage map based on new, transcriptome-anchored markers; (ii) map four important agronomic traits, namely, vernalization requirement, seed alkaloid content, and resistance to anthracnose and Phomopsis stem blight; and, (iii) define regions of synteny between the L. albus and narrow-leafed lupin (L. angustifolius L.) genomes. Mapping of white lupin quantitative trait loci (QTLs) revealed polygenic control of vernalization responsiveness and anthracnose resistance, as well as a single locus regulating seed alkaloid content. We found high sequence collinearity between white and narrow-leafed lupin genomes. Interestingly, the white lupin QTLs did not correspond to previously mapped narrow-leafed lupin loci conferring vernalization independence, anthracnose resistance, low alkaloids and Phomopsis stem blight resistance, highlighting different genetic control of these traits. Our suite of allele-sequenced and PCR validated markers tagging these QTLs is immediately applicable for marker-assisted selection in white lupin breeding. The consensus map constitutes a platform for synteny-based gene cloning approaches and can support the forthcoming white lupin genome sequencing efforts.

Highlights

A high-density consensus linkage map of white lupin highlights synteny with narrow-leafed lupin and provides markers tagging key agronomic traits
White lupin (Lupinus albus L., WL) is a legume crop cultivated in ancient Greece and Egypt more than three thousand years ago[1]
The objectives of this study were (1) to construct a new consensus linkage map of WL integrating those previously published[11,31] with a rich set of newly developed, sequence-defined markers anchored to the species transcriptome; (2) to provide high-resolution QTL assay of anthracnose resistance, vernalization responsiveness and low alkaloid content, and (3) to investigate synteny between WL and its sister lupin crop, NLL

Summary

Introduction

A high-density consensus linkage map of white lupin highlights synteny with narrow-leafed lupin and provides markers tagging key agronomic traits. This study aimed to (i) develop a new highdensity consensus linkage map based on new, transcriptome-anchored markers; (ii) map four important agronomic traits, namely, vernalization requirement, seed alkaloid content, and resistance to anthracnose and Phomopsis stem blight; and, (iii) define regions of synteny between the L. albus and narrow-leafed lupin (L. angustifolius L.) genomes. Untapped landraces with higher yielding ability than current cultivars await thorough exploitation in breeding programmes[9] Despite such a long domestication history and considerable opportunities for crop improvement, WL breeders have to struggle with various challenges hampering worldwide cultivation of the species that include, inter alia, the exploitation of optimal vernalization requirement for floral initiation and the identification of genetic resistance to devastating diseases, such as Institute of Plant Genetics Polish Academy of Sciences, Strzeszyńska 34, 60-479, Poznań, Poland.

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