Abstract
A rapid, sensitive and selective analysis method using Ultra High Performance Liquid Chromatography coupled to triple-quadrupole Mass Spectrometry (UHPLC-QqQ-MS) has been developed for the quantification of polyphenols in rosé wines. The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, the present method allows the selective quantification of up to 152 phenolic and two additional non-phenolic wine compounds in 30 min without sample purification or pre-concentration, even at low concentration levels. This method was repeatably applied to a set of 12 rosé wines and thus proved to be suitable for high-throughput and large-scale metabolomics studies.
Highlights
Rosé is a highly diversified type of wine widely produced and consumed worldwide
Low-concentrated compounds which were not detected by UHPLC-Ion Trap (IT)-Mass Spectrometry (MS) were targeted by UHPLC-QqQ-Multiple Reaction Monitoring (MRM) based on transitions reported in literature
MRM transitions parameters for each compound were optimised on the UHPLC-QqQ-MS system and compared to data reported in the literature [5,6,14,16,17,22,25,26,28,29,30,31,32,33,34,35,44,45,46]
Summary
Rosé is a highly diversified type of wine widely produced and consumed worldwide. Polyphenols are key components of wines, as they are responsible for colour, taste and the quality of the wines. The use of mass spectrometry coupled to (U)HPLC-DAD techniques has brought significant improvements in polyphenol quantification, mainly by reducing analysis times and increasing sensitivity. A comparative study performed by Spáčil et al [21] on the quantitative analysis in grape, wines and tea of 34 phenolic substances divided into four categories highlights the efficiency of the use of QqQ-MS and UHPLC These four classes of compounds were analysed separately. The present work challenges this conclusion by describing a high-throughput UHPLC-QqQ-MS quantification method for rosé wine polyphenol analysis This method was based on Multiple Reaction Monitoring (MRM) detection mode in order to increase both selectivity and sensitivity while reducing analysis times. The suitability of this method for large-scale wine analyses and metabolomics studies was demonstrated on a set of 12 rosé wines
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