Abstract
Innate immunity is at the frontline of defense against pathogens. Macrophages are a group of phagocytes that secrete cytokines to mediate the transmission of information on pathogen infectiousness to the acquired immune system. Our previous study established an assay for quantifying phagocytic activity in two human monocytic cell lines, U937 and THP-1. Overall, this assay system was quick, easy, and robust, except for the time-consuming quantification step using image analysis. Thus, in this study, we improved our previous assay system. For evaluating phagocytic activity, fluorescently labeled magnetic beads were phagocytosed and detected with a fluorescent-multi well plate reader. This system facilitates high-throughput screening of macrophage activating factors.
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