Abstract
An IF‐M3‐deficient ascites cell‐free system is described. An IF‐M3 species of high molecular weight has been isolated and partially purified from a high‐KCl ascites ribosomal wash fluid. Translation of encephalomyocarditis virus RNA and globin mRNA is completely dependent upon the presence of the ascites IF‐M3, while the factor is not required with either poly(A,U,G) or poly(U) as messengers. The sparsomycin technique for the generation of short initiation peptides has been employed to demonstrate that IF‐M3 acts at the level of chain initiation. Rabbit reticulocyte IF‐M3 and IF‐E3, as well as rabbit liver IF‐M3, can substitute for ascites IF‐M3 in EMC‐RNA and globin mRNA translation. The translation of EMC‐RNA in the ascites cell‐free system is, therefore, dependent upon the presence of two discrete initiation factors, a high‐molecular‐weight species, IF‐M3, and a smaller component, IFEMC.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
