Abstract
Green analytical technologies for the determination of a bioactive compound diosmin (DIOM) in the real samples of pharmaceutical formulations and biological fluids are scarce in literature. Therefore, the present investigation was carried out to develop a novel, rapid, simple, and economical green “reversed phase high-performance thin-layer chromatography (RP-HPTLC)” method for the determination of DIOM in commercial tablets and in-house developed spray-dried microparticles (MPs). The quantification of DIOM was conducted via “RP-18 silica gel 60 F254S HPTLC plates”. The binary combination of green solvents, i.e., ethanol:water (5.5:4.5 v/v) was proposed as a green mobile phase. The analysis of DIOM was conducted in absorbance/reflectance mode of densitometry at λmax = 348 nm. The densitograms of DIOM from the commercial tablets and in-house developed spray-dried MPs were verified by recording their single band at Rf = 0.80 ± 0.02 compared to standard DIOM. Green RP-HPTLC method was observed as linear in the range of 100–700 ng/band with R2 = 0.9995. The proposed method was found as “accurate, precise, robust, and sensitive” for the determination of DIOM in the real samples of commercial tablets and in-house developed spray-dried MPs. The % content of DIOM in the real samples of commercial tablets and in-house developed spray-dried MPs was obtained as 99.06 and 101.30%, respectively. The recorded results of this research suggested that the green RP-HPTLC method can be effectively used for the routine analysis of DIOM in pharmaceutical products.
Highlights
Flavonoids are reported as an important class of bioactive compounds which are obtained from various medicinal plants [1]
These results suggested that high-performance thin layer chromatography (HPTLC) spots of DIOM were developed well under the chamber saturation condition using a green mobile phase
Minor variations values and lower values f indicated that that green green RP-HPTLC
Summary
Flavonoids are reported as an important class of bioactive compounds which are obtained from various medicinal plants [1]. Several “high-performance liquid chromatography (HPLC)” methods coupled with UV detector have been reported for the determination of DIOM in pharmaceutical preparations either alone or in combination with other bioflavonoids [1,19,20,21,22]. The utility of silver nanoparticles was tried in the analysis of DIOM in plant extracts and pharmaceutical dosage forms [24]. A single “high-performance thin layer chromatography (HPTLC)” method has been reported for the determination of DIOM in different citrus fruit extracts and pharmaceutical dosage forms [29]. Different techniques have been reported for the determination of DIOM in plant extracts and pharmaceutical dosage forms, but none of them are green analytical technique. The utility of the proposed RP-HPTLC method was verified in the analysis of DIOM in the real samples of commercial tablets and in-house developed spray-dried microparticles (MPs)
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