Abstract
A rapid and simple immuno-chromatographic assay was developed to detect aflatoxin B1 (AFB1). The assay was based on a modified competitive binding format using colloidal gold and polyclonal antibody (Pab) conjugates. The anti-AFB1 Pab was immobilized to a defined detection zone on a porous nitrocellulose membrane and colloidal gold particles were conjugated to AFB1-BSA which served as a detection reagent. The AFB1-containing sample was added to the membrane and allowed to move with AFB1-BSA-coated particles dried on the conjugation pad. The mixture was then passed along the porous membrane by capillary action past the Pab in the detection zone, which captured AFB1 or AFB1-BSA. AFB1 in the sample inhibits binding of AFB1-BSA conjugated gold particles to the Pab and prevents formation of a red color dot. In the absence of AFB1, AFB1-BSA conjugated gold particles bound to the Pab, give a red color within this detection zone. With this method, 10 μg/mL of AFB1 was detected in less than 10 min. The developed AFB1 assay also showed no cross reaction to Ochratoxin A (OTA).
Highlights
Aflatoxin B1 (AFB1), produced by Aspergillus flavus and A. parasiticus, is one of the most poisonous toxins in the world
Applicability is improved in developing multi strip assays for detection of multiple mycotoxins
Only AFB1-polyclonal antibody (PAb) was used to probe on the strip
Summary
Aflatoxin B1 (AFB1), produced by Aspergillus flavus and A. parasiticus, is one of the most poisonous toxins in the world. Exposure to this toxin induces higher risk of the incidence of hepatocellular carcinomas [1]. AFB1 could be produced in a number of agricultural products under favorable growth conditions (temperature, humidity, and pressure) during storage [3]. AFB1 is known to occur naturally in agricultural products such as peanuts, corn, and animal feeds [4]. These contaminated food or feeds present serious health hazards [1]. In the European Union, the maximum residue levels (MRLs) are 2 μg/kg in nuts and dried fruits, and
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