Abstract
Determination of Staphylococcus aureus was performed by using a piezoelectric crystal immunosensor system. The system consists of an oscillator, a frequency counter, a special cell and a modified piezoelectric crystal. Goat-anti-human IgG is immobilized on the surface of the crystal, which is used as a S. aureus detector by measuring its resonant frequency shift due to a mass change caused by specific binding of the microbe to the crystal surface. The frequency shift was correlated with a S. aureus concentration in the range of 5 × 10 5−1 × 10 8 cells. The coated crystal was stable for fifteen assays without detectable loss of sensitivity and stable for a month when it was stored at +4°C.
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