A γ-glutamyl transpeptidase of Aphidius ervi venom induces apoptosis in the ovaries of host aphids

Abstract

Parasitism by the endophagous braconid Aphidius ervi (Hymenoptera, Braconidae) has a negative impact on the reproductive activity of its host, Acyrthosiphon pisum (Homoptera, Aphididae). The host castration is induced by the parasitoid venom and is reproduced by the injection of chromatographic fractions highly enriched with two proteins, of 18 (p18) and 36 kDa (p36) in size, respectively. Here we demonstrate that these bioactive proteins trigger apoptosis of the cells in the germaria and ovariole sheath of the host aphid. Both p18 and p36 were internally sequenced and the gathered information was matched against the deduced amino acid sequence of the putative proteins encoded by cDNA clones, randomly selected from a cDNA library, which was raised using mRNA extracted from A. ervi venom glands. The identified cDNA clones contained an insert corresponding to the RNA product of an interrupted gene, made of six exons and five introns, which was found to be transcribed at higher levels in adult females of A. ervi than in males. This gene codes for a putative protein composed of 541 amino acids, with a calculated molecular mass of 56.9 kDa, which contained the amino acid sequences experimentally determined for both p18 and p36. This putative protein showed a significant level of sequence identity with γ-glutamyl transpeptidases ( γ-GT), and it was named Ae- γ-GT. The γ-GTs are enzymes which play a key role in the metabolism of glutathione (GSH) and, as observed in most organisms, they are membrane-bound heterodimers formed by a large and a small subunit, which originate by post-translational processing of a single-chain precursor. The expression in insect cells of Ae- γ-GT confirmed the occurrence of the expected post-translational processing, and demonstrated that, unlike other γ-GTs, this protein is secreted in the extracellular environment. A measurable γ-GT activity was detected in the venom of A. ervi and in the chromatographic fractions containing Ae- γ-GT. Thus, we suggest that this venom protein may induce apoptosis in the host ovarioles by generating an alteration of the GSH metabolism and a consequent oxidative stress.