A glassy carbon electrode modulated with Poly (Naphthol green B) for simultaneous electroanalysis of serotonin and Epinephrine in presence of l-tryptophan

Abstract

Present study reports the simplified and efficient method for the quantification of Serotonin(5-HT)and Epinephrine(EP) by using a glassy carbon electrode customised with poly(Naphthol green B) film. The electrochemical measurements were performed through cyclic and differential pulse voltammetry. The experimental outcomes authenticates that the developed sensor accelerates the electrocatalytic current response with reduced overvoltage for 5-HT and EP. The operating system and certain experimental variables includes Sweep rate, differed pH values of supporting medium and the impact of varying concentration of 5-HT and EP were adjusted. The sweep rate study reveals the diffusion controlled kinetics for 5-HT and EP at P-NGB/MGCE. At the optimum reaction conditions, the fabricated electrode exhibits the linear behaviour in the concentration range of 0.1 × 10-4M to 0.8 × 10-4M and 0.1 × 10-4M to 1 × 10-4 M with the lower limit of detection of 2.3 μM & 1.3 μM for 5-HT and EP respectively. The selectivity of the constructed electrode was achieved by concurrent determination of 5-HT and EP in presence of l-Tryptophan(LT). This provides the advantageous of the fabricated sensor as it shows good sensibility, convenient method for fabrication and excellent selectivity for specific and simultaneous study of 5-HT and EP.